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Workshop

MS Courses by Dr. Jack Henion: Advanced LC-MS/MS (Day1) & Interpretation of MS/MS CID Spectra (Day2)


Speakers: Jack Henion, PhD (Emeritus Professor of Cornell University)
Organizers:
Date: 2020-02-24- 02/25/2020
Time: 8:45-17:00 Pacific Time
Registration fee Per Day: Regular $195; Academic $125; For unemployed or students $35; For vendor-show rep $35; For major-sponsor rep (incl lunch): $0
Location: San Francisco Bay Area: Foster City Crowne Plaza
Major Sponsor:
Vendor show vendors registered to date:
Registration: http://www.PBSS.org
Registration deadline:2020-02-24  (it will close sooner if the seating cap is reached)

About the Topic

Day 1: Advanced LC/MS Practices

Course Objective:

Learn effective techniques for analyzing complex samples and characterizing organic compounds

Key Topics You'll Learn About

  • Principles and preferred practices for APCI LC/MS
  • Fundamentals and practices for optimal ESI (electrospray ionization)
  • Principles of multiple charging in electrospray mass spectra of biomolecules
  • Strategies for characterizing small molecules contained in biological samples via electrospray LC/MS and LC/MS/MS.
  • Sample preparation optimized for LC/MS and LC/MS/MS analyses
  • Initial strategies for interpreting collision-induced dissociation (CID) mass spectra of small pharmaceutical molecules.

 
Day 2: Interpretation of MS/MS CID Mass Spectra

 This relatively advanced course covers basic concepts for interpreting MS/MS collision-induced dissociation (CID) mass spectra of small molecules such as drugs, metabolites, impurities, degradants, environmental contaminants, etc.  This is a one-day course with six one-hour lectures including extensive problem solving.  The prospective student should have a good understanding of organic chemistry, reaction mechanisms, and the guidelines of physical organic chemistry.  An understanding of EI mass spectral interpretation techniques will also be helpful.

Key Topics You Will Learn

  • Strategies for approaching CID mass spectral interpretation
  • The importance of acquiring high-quality CID mass spectral information for interpretation purposes
  • The nitrogen rule, importance of stable isotope contributions, mass defects, the Retro Diels Alder reaction, accurate mass measurement, high resolution and related topics.
  • Differentiation between simple bond cleavages, alpha cleavages, rearrangements, and ring opening mechanisms
  • Rules for approaching an unknown structure characterization given a good CID mass spectrum
  • Relevance of data from triple quadrupole MS systems, Ion Trap MS, LC-TOF/MS, FTMS, Orbitraps, MSn and other modern analytical information 

 

Further Details:

Day 1:

Who Should Attend

Experienced LC/MS researchers and practitioners or others involved in identifying or measuring organic compounds in real-world samples including drugs and metabolites within or for the pharmaceutical industry, and who work with HPLC and MS techniques.  Prior HPLC and/or LC/MS experience and understanding is assumed.

Overview of the course

Atmospheric Pressure Chemical Ionization (APCI)

  • Mechanism of APCI
  • Basic principles
  • Gas-phase chemistries 
  • Applications
  • Problem solving

Electrospray Introduction

  • Basic principles and mechanism of electrospray
  • Applications
  • Techniques for success
  • Quantitative analysis applications
  • Application problems

Electrospray of Large Molecules

  • Basic principles
  • Multiple charging
  • Determination of high molecular weights
  • Peptide and protein characterization
  • Biospecific non covalent interactions
  • Bottom up vs. Top down bioanalysis
  • Examples of both bottom-up and top-down protein bioanalysis

Electrospray of Small Molecules

  • Basic principles and strategies
  • Applications
  • Quantitative analysis applications
  • Challenging issues related to electrospray ionization
  • Applications problems

Sample Preparation for LC/MS

  • Types of extraction
  • Sample types
  • LSE
  • SPE
  • Immunoaffinity isolation of targeted compounds for bioanalysis
  • 96-well format sample preparation
  • Automated sample preparation—off-line, on-line
  • Micro sampling, dried blood spots, micro capillary collection, VAMS
  • High-throughput analysis strategies

Recent Developments in Mass Spectrometry

  • Mass Analyzers
  • Compact mass spectrometers
  • Alternative ionization modes
  • Nano electrospray
  • Ambient ionization
    • DART
    • DESI
    • ASAP
  • UPLC/MS/MS
  • Differential mobility spectrometry
  • Role of mass spectrometry in the cannabis industry

 
Day 2:

Who Should Attend

Researchers, practitioners, or others involved in identifying compounds detected in real-world samples including drugs, metabolites and toxicants.  Scientists who are interested in the agrochemical, industrial, toxicological or chemical fields will also benefit from this course, although the focus is on small molecule pharmaceutical compounds and problems. Some examples of the cannabinoid structure characterization will also be included given the new growing interest in the hemp and cannabis industry.

The course is designed as a practical, comprehensive introduction, which should enable the interested student to apply the basic rules of mass spectral interpretation for characterizing unknown compounds.  It is desirable for the student to be familiar with the basics of physical organic chemistry mechanisms, atmospheric pressure ionization principles, and tandem mass spectrometry.

How You Will Benefit from This Course

  • Gain insight to approaching your analytical problems from an experienced practitioner.
  • Review the basics and parallels between the interpretation of EI mass spectra compared with CID mass spectra
  • Learn specific LC/MS and LC/MS/MS techniques for characterizing or identifying components in complex samples
  • Perform numerous problem solving exercises from applications in the environmental, biotechnology, pharmaceutical, chemical industries, and chemical terrorism including trace analyses
  • Pick up helpful suggestions and hints to help solve your own analytical problems

About the Speakers

Jack Henion is Professor Emeritus of Toxicology at Cornell University in the Analytical Toxicology Lab.  He is also a co-founder and CSO of Advion, Inc., located in Ithaca, New York.  Prof. Henion’s work in LC/MS relates to the analysis of real-world samples common to pharmaceutical, environmental, and biochemical problems.  The instructor has published extensively in the areas of conventional capillary GC/MS as well as LC/MS, SFC/MS, IC/MS, and CE/MS using atmospheric pressure ionization (API) technologies with quadrupole, ion trap, FTMS, and time-of-flight mass spectrometers.


2019-11-12, Antibody Drug Discovery and Development: Hit Generation, Engineering, Cell-line Development, IND enabling studies, and Biosimilars
2019-12-10, In Vitro Diagnostics (IVD), Companion Diagnostics (CDx) and Precision Medicine
2020-01-10, All about half-life: which one should I use? (tentative title)
2020-01-21, Considerations for Successful Clinical Development: Fundamentals and Best Practices for Clinical Trial Design, Biostatistics, and Data Management
2020-02-11, Development of Drug/Device Combinations
2020-03-16, Clinical Trials Essentials: An Intensive One-Week Course (register for the full week or half day sessions)
2020-04-10, Biopolymer-based conjugation of therapeutic proteins and antibodies for improving drug properties (tentative title)
2020-05-12, Regulated Large Molecule Bioanalysis: Fundamentals, the New FDA Guidance and Beyond
2020-05-22, No More Undruggables: Unconventional Approaches for Drug Discovery and Design - prodrugs, PROTACS, fragment-based design, directed evolution and beyond
2020-06-23, Advances in Immuno-Oncology (A Joint Symposium by PBSS and BioScience Forum)
┬ęPharmaceutical & BioScience Society, International; Last Modified: 10/20/2019; Admin Logon
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